Полимеразная цепная реакция

Молекулярная генетика. Генная инженерия
Полимеразная цепная реакция

Некоторые методы генной инженерии приобретают все более важное значение в клинической практике.

 
А. Полимеразная цепная реакция

Полимеразная цепная реакция [ПЦР (PCR)] позволяет многократно воспроизводить (амплифицировать) выбранный фрагмент ДНК без помощи рестриктаз, векторов или клетки-хозяина (см. Клонирование ДНК). Для этого нужно иметь в своём ...

А. Полимеразная цепная реакция
Б. Анализ длины рестрикционных фрагментов

Рестриктазы расщепляют ДНК в специфических участках, обычно в палиндромных последовательностях (см. Клонирование ДНК). Когда одно из оснований в такой последовательности изменяется в результате мутации, этот участок перестаёт ...

Б. Анализ длины рестрикционных фрагментов
В. Сверхэкспрессия белков

Для лечения тех или иных заболеваний необходимы белки, например белковые гормоны, которые у животных присутствуют в малых количествах и поэтому труднодоступны. В настоящее время такие белки можно получать ...

В. Сверхэкспрессия белков

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