 | Название: | NMR Studies of Structural Motifs: Protein Folding and Ligand Binding |
| Автор: | Christine Sallum | |
| Год: | 2010 | |
| ISBN: | 3838335775 | |
| Количество страниц: | 176 | |
| Цена: | 9 975 руб. | |
| Описание: | NMR of Structural Motifs: The agrin G3 domain is critical in development and maintenance of the neuromuscular junction. G3 binds -dystroglycan and initiates acetylcholine receptor clustering on myotube membranes. Using NMR spectroscopy, we show both active B8 and inactive B0 isoforms binding sialic acid, heparin, and heparan sulfate and map the binding sites. Ligand dissociation constants were determined by isothermal titration calorimetry. Pulse field gradient diffusion NMR was used to determine heparin binding stoichiometry, 2 protein per heparin. Residual dipolar couplings (RDCs) were used to probe residual denatured OB-fold protein structure; folded staphylococcal nuclease (SN) has greater RDC differences. Invariance of denatured SN RDCs had been attributed to robustness of long-range denatured state structure. We show, however, that increased RDC differences in the folded states are a consequence of close packing of native state polypeptide, more consistent with... | |
| Заказать, либо купить книгу |
Структура:
Списки:
Сложность материала:
Величины и единицы:
Книги Список книг
Books a la Carte Plus for Microbiology with Diseases by Taxonomy (3rd Edition) The Third Edition of Microbiology with Diseases by Taxonomy is the most cutting-edge microbiology book available, offering unparalleled currency, ...
Металлы, которые всегда с тобой Металлы, находящиеся в незначительных количествах внутри живого организма, ...
Biological Aging: Methods and Protocols Biological Aging: Methods and Protocols investigates the various processes that are affected by the age of an organism. Several new tools for the ...
Микроэлементы в окружающей среде. Биогеохимия, биотехнология и биоремедиация Книга посвящена анализу роли микроэлементов (металлов и металлоидов) в ...